The OmniPac? PCX-500 column is a macroporous, high-capacity, solvent-compatible
column designed to separate cationic and neutral species in a single
run. This column combines cation-exchange and polymeric reversed-phase
retention mechanisms in a single column.
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Good for the simultaneous separation of neutral and cationic species
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Acid and base compatible, pH 0–14
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100% solvent compatible
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Not as effective as the CS12A for inorganic cations
The
multimode separation mechanism is achieved by coating a macroporous
hydrophobic core for reversed-phase retention with a cation-exchange
latex bead layer for cation-exchange retention. The macroporous
structure provides an accessible hydrophobic core where reversed-phase
retention occurs. The cation-exchange selectivity is provided by a
cation-exchange MicroBead? layer that coats the hydrophobic
core. Since the cation-exchange layer is identical to that of the
OmniPac PCX-100, the two columns exhibit similar ion-exchange
selectivity. As a result, the OmniPac PXC-100 can be used to develop the
ion-exchange separation, and the method can then be transferred to the
OmniPac PXC-500 for further refinement to include solvent for the
reversed-phase separation. The OmniPac PCX-500 is 100% solvent
compatible and should always be run with at least 1% organic solvent in
the mobile phase to maintain column life.
The OmniPac PCX-500 may
be used for the separation of compounds such as adrenergics, alcohols,
anilines, antidepressants, anti-inflammatory agents, plating bath
brighteners, cephalosporins, stains and dyes, herbicides, nucleotides
and nucleosides, peptides, sulfonamides, and water-soluble vitamins.