美國(guó)戴安Dionex CarboPac PA200 Carbohydrate Column 062895 生物色譜柱

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產(chǎn)品名稱: 美國(guó)戴安Dionex CarboPac PA200 Carbohydrate Column 062895 生物色譜柱

產(chǎn)品型號(hào): 062895

產(chǎn)品展商: 戴安Dionex

產(chǎn)品文檔: 無(wú)相關(guān)文檔

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美國(guó)戴安Dionex CarboPac PA200 Carbohydrate Column 062895 生物色譜柱 的詳細(xì)介紹

Fast, pH-Stable Separation of Oligosaccharides

A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.

Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization

CarboPac^? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.

CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications
Dimensions	CarboPac^? PA200 Analytical Column: 3 × 250 mm CarboPac PA200 Guard Column: 3 × 50 mm
Maximum operating pressure	4000 psi (27.9 MPa)
Temperature range	6–60 °C Recommended operating temperature: controlled ambient
Typical operating conditions	2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min
Mobile phase compatibility	pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only.
Resin composition	5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead^? quaternary amine functionalized latex (6% crosslinked)
Anion-exchange capacity	Approximately 35 μeq/column (3 × 250 mm analytical column)
Column construction	PEEK^? with 10-32 threaded ferrule-style end fittings. All components are nonmetallic