Size-Exclusion Chromatography (SEC) Columns for Separating Water-Soluble Polymers
Thermo Scientific? Acclaim? SEC-300 and SEC-1000 columns are a family of high-performance
size-exclusion chromatography columns specifically designed for the
separation of water-soluble polymers and oligomers. Acclaim SEC columns
are available in two pore sizes, 300 ? and 1000 ?, and are optimized for
separating a broad molecular weight range. Water-soluble polymers cover
a wide range of highly varied families of products, both of natural or
synthetic origin, and have numerous uses in pharmaceuticals,
medications, cosmetics, food products, and industrial processes, to name
a few.
Column features include:
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Proprietary monodispersed, multipore, hydrophilic resin: no inflection points in calibration curve.
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Two columns with different pore sizes offer wide linear range (100 to 1,000,000 Dalton).
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Availability
of small particle sizes packed in 4.6 × 300 mm dimension allows for
high-resolution analysis with reduced solvent consumption.
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Stable surface bonding with low column bleed and compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex Corona? Charged Aerosol Detectors.
These
columns are packed with spherical, monodispersed, hydrophilic
polymethacrylate particles synthesized by multipore particle synthesis
technology. Combined with the use of small particle sizes (5 μm for
Acclaim SEC-300 and 7 μm for Acclaim SEC-1000), they provide
high-resolution and good linearity of the calibration curve for accurate
and reliable analysis of a variety of water-soluble polymers.
The
Acclaim SEC-1000 uses polymethacrylate particles with a nominal pore
size of 1000 ?, resulting in a broad linear calibration range from 1,000
to 1,000,000 Dalton. Therefore, it is suitable for characterizing high
molecular weight polymers. The Acclaim SEC-300 uses polymethacrylate
particles with a nominal pore size of 300 ?, giving a linear calibration
range from 100 to 50,000 Dalton. As a result, this column offers good
resolution for lower molecular weight polymers or oligomers.